This Friday Nov 6, 5pm - 6pm, Med school (Traylor 709)
Mohsen Mollazadeh is presenting! Title and abstract coming soon
title: Monitoring of neuronal activity during dexterous hand movements.
abstract: In the first part of the talk, I will present the VLSI circuit we have developed for monitoring neuronal activity in awake behaving studies. In the second part, I will present the study of population neuronal activity in primate's motor cortex during dexterous hand movements. I will present how LFP signals are modulated with various grasp patterns and their relationship to single unit activity.
Monday, November 2, 2009
Monday, September 21, 2009
FSSHH this Friday!!!
This Friday Sept 25th 5pm - 6pm, Med school (Traylor 709)
Kartik is presenting on 'optical techniques and VLSI systems for structural and functional brain imaging'.
See you all there!
Abstract: optical techniques for investigating the brain offer several key strengths - non-contact, minimally invasive, multi-scale (single cells to populations), functional/structural observation. currently, most small animal imaging is restricted to restrained &/or anesthetized animals. electrophysiology in awake, behaving rodents has led to many interesting behavioral results, but imaging in a similar scenario has not been explored well. i've been working on designing miniaturized optical imaging systems that incorporate illumination, optics and image sensing electronics in a small volume device that can be mounted on a rodent for chronic imaging in awake and behaving animals. i'll talk about the design and characterization of the imaging system and the image sensor and some preliminary experiments in rats.
Tuesday, September 8, 2009
Next FSSHH Friday Sept 11 2009: Sabyasachi Roy
FSSHH Returns this fall on Friday Setp 11, 2009. Sabyasachi Roy will be presenting at Talbot Library (Traylor 709)
Abstract:
Application of multi-channel telemetry in auditory neurophysiology & behavior
The neural basis of vocal control and auditory feedback has long interested neuroscientist and biomedical engineers alike. Our lab has focused on studying the cortical neural activity in the common marmoset in understanding this basic sensory-motor system. The marmoset monkeys (Callithrix Jacchus) is an ideal animal model given the fact that they remain highly vocal in captivity. This essential vocal behavior is primarily elicited when the subject is in a natural behavior state i.e. free roaming condition. Typically, neurophysiological experiments on vocal production and feedback have involved either restrained subjects or tethered setups that severely restrict the behavior of the subject. This is where multi-channel neural telemetry can play a vital role of enabling the vocal behavior of primates during auditory experiments and providing the same quality of neural data as tethered setups. We are developing the essential wireless radio link as well as the backend processing capability to make this technology practical and effective. These small, lightweight wireless devices can continuously transmit multiple channels of neural data from the relevant cortical area of the marmoset while it is engaged in an experimental task. I will briefly highlight our progress in developing custom telemetry system and integrating existing devices as well as the experimental opportunities that this technology opens up.
Abstract:
Application of multi-channel telemetry in auditory neurophysiology & behavior
The neural basis of vocal control and auditory feedback has long interested neuroscientist and biomedical engineers alike. Our lab has focused on studying the cortical neural activity in the common marmoset in understanding this basic sensory-motor system. The marmoset monkeys (Callithrix Jacchus) is an ideal animal model given the fact that they remain highly vocal in captivity. This essential vocal behavior is primarily elicited when the subject is in a natural behavior state i.e. free roaming condition. Typically, neurophysiological experiments on vocal production and feedback have involved either restrained subjects or tethered setups that severely restrict the behavior of the subject. This is where multi-channel neural telemetry can play a vital role of enabling the vocal behavior of primates during auditory experiments and providing the same quality of neural data as tethered setups. We are developing the essential wireless radio link as well as the backend processing capability to make this technology practical and effective. These small, lightweight wireless devices can continuously transmit multiple channels of neural data from the relevant cortical area of the marmoset while it is engaged in an experimental task. I will briefly highlight our progress in developing custom telemetry system and integrating existing devices as well as the experimental opportunities that this technology opens up.
Saturday, August 15, 2009
Need volunteers for Fall 2009!
please email luke ( lukejohnson07 AT gmail.com) and/or Yoonju ( cho.yoonju AT jhmi.edu) to volunteer!
What is the Friday Student Seminar and Happy Hour?
FSSHH is a regularly scheduled seminar series for Johns Hopkins BME grad students and other interested students that includes a seminar talk, happy hour refreshments (beer, soda appetizers), and fun social interactions.
Why have it?
It has gives students opportunities to present their work and learn about their peers research in a relaxed environment. It also is a great opportunity for students to socialize and meet students from other labs and years.
When is it?
One or two Fridays a month @ 5pm
Where is it?
Either Homewood Clark 110, or 7th floor Library in Traylor (Talbot) at the Med Campus
What will go on?
Generally, the first 15 - 30 minutes will be spent drinking, eating snacks, etc, and the last 20-30 minutes there will be a talk by a fellow grad student. The nature of the talk is completely up to the presenter, but the hope is that they wont be overly technical, so that anybody from our very diverse department could listen and be interested and at least somewhat understand.
Questions/Comments? email lukejohnson07 AT gmail.com or cho.yoonju AT jhmi.edu
What is the Friday Student Seminar and Happy Hour?
FSSHH is a regularly scheduled seminar series for Johns Hopkins BME grad students and other interested students that includes a seminar talk, happy hour refreshments (beer, soda appetizers), and fun social interactions.
Why have it?
It has gives students opportunities to present their work and learn about their peers research in a relaxed environment. It also is a great opportunity for students to socialize and meet students from other labs and years.
When is it?
One or two Fridays a month @ 5pm
Where is it?
Either Homewood Clark 110, or 7th floor Library in Traylor (Talbot) at the Med Campus
What will go on?
Generally, the first 15 - 30 minutes will be spent drinking, eating snacks, etc, and the last 20-30 minutes there will be a talk by a fellow grad student. The nature of the talk is completely up to the presenter, but the hope is that they wont be overly technical, so that anybody from our very diverse department could listen and be interested and at least somewhat understand.
Questions/Comments? email lukejohnson07 AT gmail.com or cho.yoonju AT jhmi.edu
Thursday, June 4, 2009
-- Yi Zhang
We performed on-chip DNA methylation analysis using methylation-specific PCR (MSP) within an arrayed micro droplet-in-oil platform that is designed for more practical application of microfluidic droplet technologies in clinical applications. Unique features of this ready-to-use device include arrayed primers that are pre-deposited into micro-reaction chambers and use of the oil phase as a companion fluid for both sample actuation and compartmentalization. These technical advantages allow for infusion of minute amounts of sample for arrayed MSP analysis, without the added complexities inherent in microfluidic droplet-based studies. Ease of use of this micro device is exemplified by analysis of two tumor suppressor promoters, p15 and TMS1 using an on-chip methylation assay. These results were consistent with standard MSP protocols, yet the simplicity of the droplet-in-oil microfluidic PCR platform provides and easy and efficient tool for DNA methylation analysis in a large-scale arrayed manner.
Tuesday, May 12, 2009
May 15, 2009; Homewood, Clark 110
-- Chris Puleo
Abstract: The long term goal of this proposal is to develop microfluidic technologies that enable the widespread use of confocal fluorescence spectroscopy (CFS) for single molecule detection (SMD) in vital applications including, single molecule diagnostics and single cell analysis. Currently amplification techniques have been used to determine the presence of rare biomolecules in such applications; however, the cost, complexity, and time requirements associated with these tests limit clinical value and general utility. Direct molecule-by-molecule assessment using SMD remains an intriguing replacement for amplification technologies due to high sensitivity, assay simplicity, and low costs; however, technical challenges continue to limit the utility of these single molecule techniques. We use microfluidic interfaces for SMD platforms to expand applicability in the analysis of rare molecules from complex biological fluids. The overall goal of this work is apply microfluidics to SMD assays in order to obtain the lowest possible detection limits from the smallest possible sample volume. Direct implications are clear, high-throughput biomolecular analysis from the most precious and rare biological samples. In theory, single molecule sensitivity confers infinite detection limits in CFS platforms; however, in practice SMD platforms rely on continuous flow formats and bulk probe-target reactions. These design flaws yield significant fundamental pitfalls and result in practical limitations: 1) Analyte delivery to the microscale sensing elements is wasteful, leading to extremely low measurement efficiencies and the need for excessive sample volumes. 2) Passive probe-target interactions result in slow reaction kinetics and prohibitive assay run times. 3) Unoptimized and manual processing of target molecules results in low molecular throughput and incompatibility with arrayed formats. Experiments will be performed using two microfluidic platforms, multilayer soft lithography and water-in-oil droplets. Coupling these discrete volume control technologies with SMD provides direct control over probe hybridization, efficient transfer of target molecules to optical detection volumes, and automated processing of large numbers of sample in relatively short assay times. These improvements serve to open the door to practical use of single molecule assays in areas of vital need, such as, non-invasive diagnostic screening and investigation of cell-to-cell heterogeneity. Our lab stands in a unique position to take advantage of this potential due to practical experience in all three aspects of this challenge, including single molecule probe design, microfluidic device design and fabrication, and optical CFS platform engineering.
-- Chris Puleo
Title: "Accessible Single Molecule Detection Technologies: Microfluidic Interfaces
for Diagnostic and Single Cell Applications"
for Diagnostic and Single Cell Applications"
Sunday, April 12, 2009
FSSHH still in water!
Need a pregame(?) before the cruise?
This Friday 4/17 Hannah will be presenting at Homewood Clark 110 from 5:00 though 6:00pm (5:00 for food and 5:30 for the talk). We still need a volunteer for food and drinks, so if you are interested in it, please contact me (cho.yoonju@jhmi.edu) or Luke (luke.johnson@jhu.edu).
This is going to be AWESOME so just come and enjoy some 1st light dinner for the day before your 2nd light dinner at the cruise :)
See you all there!
April 17, 2009; Homewood, Clark 110
-- Hannah Carter
In cancer, normal cells are transformed through accumulated genetic alterations that confer a selective advantage. Somatic missense mutations are one form of genetic aberration that contribute to tumor initiation and progression. Each tumor harbors a set of these mutations, but it is unclear which are drivers, causally associated with the tumor's progression, and which are passengers, neutral in the context of selective advantage for the cancer cell. We have applied modern machine learning techniques to develop a high-throughput method for discriminating between driver and passenger somatic missense mutations identified in large scale tumor sequencing studies.
This Friday 4/17 Hannah will be presenting at Homewood Clark 110 from 5:00 though 6:00pm (5:00 for food and 5:30 for the talk). We still need a volunteer for food and drinks, so if you are interested in it, please contact me (cho.yoonju@jhmi.edu) or Luke (luke.johnson@jhu.edu).
This is going to be AWESOME so just come and enjoy some 1st light dinner for the day before your 2nd light dinner at the cruise :)
See you all there!
April 17, 2009; Homewood, Clark 110
-- Hannah Carter
In cancer, normal cells are transformed through accumulated genetic alterations that confer a selective advantage. Somatic missense mutations are one form of genetic aberration that contribute to tumor initiation and progression. Each tumor harbors a set of these mutations, but it is unclear which are drivers, causally associated with the tumor's progression, and which are passengers, neutral in the context of selective advantage for the cancer cell. We have applied modern machine learning techniques to develop a high-throughput method for discriminating between driver and passenger somatic missense mutations identified in large scale tumor sequencing studies.
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