Presenter: Susan Thompson
Title: Human Embryonic Stem Cell Derived Cardiomyocytes Ameliorate Vulnerability for Arrhythmias in an In Vitro Model of Cardiac Fibrosis
Abstract: Human embryonic stem cells (hESCs) are an attractive candidate for cardiac regeneration because of their potential to supply a large number of differentiated cardiomyocytes that can integrate into the host tissue, thereby replacing the myocytes lost during myocardial aging, disease or damage. Numerous studies have already demonstrated improved myocardial function with grafts of human embryonic stem cell-derived cardiomyocytes (hESC-CMs), alluding to possible roles of paracrine effects and direct myocardial regeneration. However, little attention has been given to the electrophysiological benefit that these cells may have on diseased myocardium, and specifically, on cardiac fibrosis, a pathological condition found in aging, heart failure, and myocardial infarction. We therefore set out to characterize the electrophysiological benefits of adding hESC-CMs to our previously reported, in vitro model of cardiac fibrosis. Following engraftment of hESC-CMs from beating hESC-CMs, LCV and TCV of fibrotic monolayers increased to 39.3±2.7 and 12.5±1.3 cm/s, respectively (n=6). In contrast, addition of hESCs from non-beating EBs suppressed LCV and TCV to 6.5±1.3 and 2.1±1 cm/s, respectively (n=4). We show for the first time that hESC-CMs reverse the loss of conduction velocity and reduce the incidence of spiral waves in an in vitro fibrosis model. This finding is significant in that it suggests that specifically hESC-CMs can directly participate in electrical propagation, perhaps through gap junction coupling, and can ameliorate abnormal conduction in fibrotic myocardium.
Wednesday, March 24, 2010
Friday, December 4, 2009
Last FSSHH of this year: Jacob Koskimaki
Last FSSHH of this year!!!!
Come enjoy Jacob's presentation, free food + drinks and awesome company of fellow BMEers!
Dec 11th (Friday) 5pm at Clark 110.
Title & abstract: Peptide optimization strategies: merging experimental data with bioinformatics tools to develop potent inhibitors of angiogenesis in breast cancer
The emergence of genomics, proteomics and new peptidomics has provided several advances in the data available to develop endogenous regulators of angiogenesis. Angiogeneis, or neovascularization, is the process where new vessels form from a preexisting microvasculature, and involves interactions among several cell types. Tumors require a blood supply to grow; similarly, abrogating this blood supply is an emerging paradigm to treat diseases such as breast cancer. Our laboratory recently developed a systematic bioinformatics-based methodology to identify several endogenous regulators of angiogenesis, and experimentally verified their activity in vitro and in vivo breast xenograft models. We now have begun to optimize these sequences to enhance activity, and show different experimental techniques combined with bioinformatics tools to increase activity of endogenous therapeutics.
The emergence of genomics, proteomics and new peptidomics has provided several advances in the data available to develop endogenous regulators of angiogenesis. Angiogeneis, or neovascularization, is the process where new vessels form from a preexisting microvasculature, and involves interactions among several cell types. Tumors require a blood supply to grow; similarly, abrogating this blood supply is an emerging paradigm to treat diseases such as breast cancer. Our laboratory recently developed a systematic bioinformatics-based methodology to identify several endogenous regulators of angiogenesis, and experimentally verified their activity in vitro and in vivo breast xenograft models. We now have begun to optimize these sequences to enhance activity, and show different experimental techniques combined with bioinformatics tools to increase activity of endogenous therapeutics.
Monday, November 2, 2009
FSSHH Friday Nov 6
This Friday Nov 6, 5pm - 6pm, Med school (Traylor 709)
Mohsen Mollazadeh is presenting! Title and abstract coming soon
title: Monitoring of neuronal activity during dexterous hand movements.
abstract: In the first part of the talk, I will present the VLSI circuit we have developed for monitoring neuronal activity in awake behaving studies. In the second part, I will present the study of population neuronal activity in primate's motor cortex during dexterous hand movements. I will present how LFP signals are modulated with various grasp patterns and their relationship to single unit activity.
Mohsen Mollazadeh is presenting! Title and abstract coming soon
title: Monitoring of neuronal activity during dexterous hand movements.
abstract: In the first part of the talk, I will present the VLSI circuit we have developed for monitoring neuronal activity in awake behaving studies. In the second part, I will present the study of population neuronal activity in primate's motor cortex during dexterous hand movements. I will present how LFP signals are modulated with various grasp patterns and their relationship to single unit activity.
Monday, September 21, 2009
FSSHH this Friday!!!
This Friday Sept 25th 5pm - 6pm, Med school (Traylor 709)
Kartik is presenting on 'optical techniques and VLSI systems for structural and functional brain imaging'.
See you all there!
Abstract: optical techniques for investigating the brain offer several key strengths - non-contact, minimally invasive, multi-scale (single cells to populations), functional/structural observation. currently, most small animal imaging is restricted to restrained &/or anesthetized animals. electrophysiology in awake, behaving rodents has led to many interesting behavioral results, but imaging in a similar scenario has not been explored well. i've been working on designing miniaturized optical imaging systems that incorporate illumination, optics and image sensing electronics in a small volume device that can be mounted on a rodent for chronic imaging in awake and behaving animals. i'll talk about the design and characterization of the imaging system and the image sensor and some preliminary experiments in rats.
Tuesday, September 8, 2009
Next FSSHH Friday Sept 11 2009: Sabyasachi Roy
FSSHH Returns this fall on Friday Setp 11, 2009. Sabyasachi Roy will be presenting at Talbot Library (Traylor 709)
Abstract:
Application of multi-channel telemetry in auditory neurophysiology & behavior
The neural basis of vocal control and auditory feedback has long interested neuroscientist and biomedical engineers alike. Our lab has focused on studying the cortical neural activity in the common marmoset in understanding this basic sensory-motor system. The marmoset monkeys (Callithrix Jacchus) is an ideal animal model given the fact that they remain highly vocal in captivity. This essential vocal behavior is primarily elicited when the subject is in a natural behavior state i.e. free roaming condition. Typically, neurophysiological experiments on vocal production and feedback have involved either restrained subjects or tethered setups that severely restrict the behavior of the subject. This is where multi-channel neural telemetry can play a vital role of enabling the vocal behavior of primates during auditory experiments and providing the same quality of neural data as tethered setups. We are developing the essential wireless radio link as well as the backend processing capability to make this technology practical and effective. These small, lightweight wireless devices can continuously transmit multiple channels of neural data from the relevant cortical area of the marmoset while it is engaged in an experimental task. I will briefly highlight our progress in developing custom telemetry system and integrating existing devices as well as the experimental opportunities that this technology opens up.
Abstract:
Application of multi-channel telemetry in auditory neurophysiology & behavior
The neural basis of vocal control and auditory feedback has long interested neuroscientist and biomedical engineers alike. Our lab has focused on studying the cortical neural activity in the common marmoset in understanding this basic sensory-motor system. The marmoset monkeys (Callithrix Jacchus) is an ideal animal model given the fact that they remain highly vocal in captivity. This essential vocal behavior is primarily elicited when the subject is in a natural behavior state i.e. free roaming condition. Typically, neurophysiological experiments on vocal production and feedback have involved either restrained subjects or tethered setups that severely restrict the behavior of the subject. This is where multi-channel neural telemetry can play a vital role of enabling the vocal behavior of primates during auditory experiments and providing the same quality of neural data as tethered setups. We are developing the essential wireless radio link as well as the backend processing capability to make this technology practical and effective. These small, lightweight wireless devices can continuously transmit multiple channels of neural data from the relevant cortical area of the marmoset while it is engaged in an experimental task. I will briefly highlight our progress in developing custom telemetry system and integrating existing devices as well as the experimental opportunities that this technology opens up.
Saturday, August 15, 2009
Need volunteers for Fall 2009!
please email luke ( lukejohnson07 AT gmail.com) and/or Yoonju ( cho.yoonju AT jhmi.edu) to volunteer!
What is the Friday Student Seminar and Happy Hour?
FSSHH is a regularly scheduled seminar series for Johns Hopkins BME grad students and other interested students that includes a seminar talk, happy hour refreshments (beer, soda appetizers), and fun social interactions.
Why have it?
It has gives students opportunities to present their work and learn about their peers research in a relaxed environment. It also is a great opportunity for students to socialize and meet students from other labs and years.
When is it?
One or two Fridays a month @ 5pm
Where is it?
Either Homewood Clark 110, or 7th floor Library in Traylor (Talbot) at the Med Campus
What will go on?
Generally, the first 15 - 30 minutes will be spent drinking, eating snacks, etc, and the last 20-30 minutes there will be a talk by a fellow grad student. The nature of the talk is completely up to the presenter, but the hope is that they wont be overly technical, so that anybody from our very diverse department could listen and be interested and at least somewhat understand.
Questions/Comments? email lukejohnson07 AT gmail.com or cho.yoonju AT jhmi.edu
What is the Friday Student Seminar and Happy Hour?
FSSHH is a regularly scheduled seminar series for Johns Hopkins BME grad students and other interested students that includes a seminar talk, happy hour refreshments (beer, soda appetizers), and fun social interactions.
Why have it?
It has gives students opportunities to present their work and learn about their peers research in a relaxed environment. It also is a great opportunity for students to socialize and meet students from other labs and years.
When is it?
One or two Fridays a month @ 5pm
Where is it?
Either Homewood Clark 110, or 7th floor Library in Traylor (Talbot) at the Med Campus
What will go on?
Generally, the first 15 - 30 minutes will be spent drinking, eating snacks, etc, and the last 20-30 minutes there will be a talk by a fellow grad student. The nature of the talk is completely up to the presenter, but the hope is that they wont be overly technical, so that anybody from our very diverse department could listen and be interested and at least somewhat understand.
Questions/Comments? email lukejohnson07 AT gmail.com or cho.yoonju AT jhmi.edu
Thursday, June 4, 2009
-- Yi Zhang
We performed on-chip DNA methylation analysis using methylation-specific PCR (MSP) within an arrayed micro droplet-in-oil platform that is designed for more practical application of microfluidic droplet technologies in clinical applications. Unique features of this ready-to-use device include arrayed primers that are pre-deposited into micro-reaction chambers and use of the oil phase as a companion fluid for both sample actuation and compartmentalization. These technical advantages allow for infusion of minute amounts of sample for arrayed MSP analysis, without the added complexities inherent in microfluidic droplet-based studies. Ease of use of this micro device is exemplified by analysis of two tumor suppressor promoters, p15 and TMS1 using an on-chip methylation assay. These results were consistent with standard MSP protocols, yet the simplicity of the droplet-in-oil microfluidic PCR platform provides and easy and efficient tool for DNA methylation analysis in a large-scale arrayed manner.
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